5 2021 Anseth Undergraduate Research Sessions

5.1 Session 2: Photoclick Hydrogels

Dr. Laura Macdougall

2021-01-26

Session 2 Article

(SI)

Topics covered

  • Using absorbance and NMR to monitor reactant conversion and product formation
  • In situ rheology, photocrosslinking, and photooxidation
  • Irradiation requirements for transdermal vs. transparenchymal phototherapy
  • Importance of validation on all Fitzpatrick skin types (avoiding racial bias in experimental design)
  • Live/dead staining to verify cytocompatability (and limitations of this approach)

Key finding: Dihydrotetrazine can be photooxidized by methylene blue and red (625 nm) light. This oxidation converts dihydrotetrazine to tetrazine, which participates in an inverse electron-demand Diels-Alder addition to norbornene (one of many “click” reactions). Because this system is activated with red light (which can penetrate into tissues that aren’t accessible with transdermal UV light), the authors demonstrated both cytocompatbility and gelation through synthetic mimics of different skin types, indicating that this material has potential for use as an injectible and dynamically stiffening hydrogel.

Additional information

Visible Light-Responsive Dynamic Biomaterials: Going Deeper and Triggering More. Rapp & DeForest. Adv Healthc Mater 2020.

Oxidation-Induced “One-Pot” Click Chemistry. Albada et al. Chem Rev 2021.

Light-Triggered Click Chemistry. Kumar & Lin. Chem Rev 2020

Photoclick Chemistry. Fairbanks et al. Chem Rev 2021

5.2 Session 3: Matrix Cues for MSC Activation

Ali Borelli

2021-01-29

Session 3 Article

(SI)

Topics covered

  • Using absorbance and NMR to monitor reactant conversion and product formation
  • In situ rheology, photocrosslinking, and photooxidation
  • Irradiation requirements for transdermal vs. transparenchymal phototherapy
  • Importance of validation on all Fitzpatrick skin types (avoiding racial bias in experimental design)
  • Live/dead staining to verify cytocompatability (and limitations of this approach)

Key finding: Dihydrotetrazine can be photooxidized by methylene blue and red (625 nm) light. This oxidation converts dihydrotetrazine to tetrazine, which participates in an inverse electron-demand Diels-Alder addition to norbornene (one of many “click” reactions). Because this system is activated with red light (which can penetrate into tissues that aren’t accessible with transdermal UV light), the authors demonstrated both cytocompatbility and gelation through synthetic mimics of different skin types, indicating that this material has potential for use as an injectible and dynamically stiffening hydrogel.

Additional information

Visible Light-Responsive Dynamic Biomaterials: Going Deeper and Triggering More. Rapp & DeForest. Adv Healthc Mater 2020.

Oxidation-Induced “One-Pot” Click Chemistry. Albada et al. Chem Rev 2021.

Light-Triggered Click Chemistry. Kumar & Lin. Chem Rev 2020

Photoclick Chemistry. Fairbanks et al. Chem Rev 2021

5.3 Session 4: Intestinal organoids in synthetic matrices

F. Max Yavitt

2021-02-01

Session 4 Article

(SI figures included in main text)

Topics covered

  • Using absorbance and NMR to monitor reactant conversion and product formation
  • In situ rheology, photocrosslinking, and photooxidation
  • Irradiation requirements for transdermal vs. transparenchymal phototherapy
  • Importance of validation on all Fitzpatrick skin types (avoiding racial bias in experimental design)
  • Live/dead staining to verify cytocompatability (and limitations of this approach)

Key finding: Dihydrotetrazine can be photooxidized by methylene blue and red (625 nm) light. This oxidation converts dihydrotetrazine to tetrazine, which participates in an inverse electron-demand Diels-Alder addition to norbornene (one of many “click” reactions). Because this system is activated with red light (which can penetrate into tissues that aren’t accessible with transdermal UV light), the authors demonstrated both cytocompatbility and gelation through synthetic mimics of different skin types, indicating that this material has potential for use as an injectible and dynamically stiffening hydrogel.

Additional information

Visible Light-Responsive Dynamic Biomaterials: Going Deeper and Triggering More. Rapp & DeForest. Adv Healthc Mater 2020.

Oxidation-Induced “One-Pot” Click Chemistry. Albada et al. Chem Rev 2021.

Light-Triggered Click Chemistry. Kumar & Lin. Chem Rev 2020

Photoclick Chemistry. Fairbanks et al. Chem Rev 2021

5.4 Session 5: MSC secretome engineering

Varsha Rao

2021-02-01

Session 5 Article

(SI)

Topics covered

  • Using absorbance and NMR to monitor reactant conversion and product formation
  • In situ rheology, photocrosslinking, and photooxidation
  • Irradiation requirements for transdermal vs. transparenchymal phototherapy
  • Importance of validation on all Fitzpatrick skin types (avoiding racial bias in experimental design)
  • Live/dead staining to verify cytocompatability (and limitations of this approach)

Key finding: Dihydrotetrazine can be photooxidized by methylene blue and red (625 nm) light. This oxidation converts dihydrotetrazine to tetrazine, which participates in an inverse electron-demand Diels-Alder addition to norbornene (one of many “click” reactions). Because this system is activated with red light (which can penetrate into tissues that aren’t accessible with transdermal UV light), the authors demonstrated both cytocompatbility and gelation through synthetic mimics of different skin types, indicating that this material has potential for use as an injectible and dynamically stiffening hydrogel.

Additional information

Visible Light-Responsive Dynamic Biomaterials: Going Deeper and Triggering More. Rapp & DeForest. Adv Healthc Mater 2020.

Oxidation-Induced “One-Pot” Click Chemistry. Albada et al. Chem Rev 2021.

Light-Triggered Click Chemistry. Kumar & Lin. Chem Rev 2020

Photoclick Chemistry. Fairbanks et al. Chem Rev 2021

5.5 Session 6: Mechanotransduction in VICs

Dilara Batan

2021-02-09

Session 6 Article

(SI)

5.6 Session 7: Organoids - Function & Characterization

Dr. Michael Blatchley

2021-02-12

Session 7 Article

(SI)

5.7 Session 8: Interpenetrating Hydrogel Networks

Bruce Kirkpatrick

2021-02-19

Session 8 Article

(SI)

5.8 Session 9: Novel LCE Biomaterial Fabrication

Nat Skillin

2021-02-23

Session 9 Article

(SI)

Additional information

Voxelated liquid crystal elastomers. Ware et al. Science 2014

5.9 Session 10: Applied Organoid Bioengineering

Ella Hushka

2021-03-01

Session 10 Article

(SI)

5.10 Session 11: Chemical Synthesis for Material Design

Joshua Kamps

2021-03-05

Session 11 Article

(SI)

5.11 Session 12: Engineered Systems for (Myo)Fibroblast Study

Cierra Walker

2021-03-09

Session 6 Article

(SI)

5.12 Session 13: Engineering Skeletal Muscle Repair

Jason Silver

2021-03-12

Session 13 Article

(SI)

5.13 Session 14: Tunable Dynamic Covalent Chemistries

Benjamin Carberry

2021-03-19

Session 14 Article

(SI)

5.14 Session 15: Micropatterning for Cellular Applications

Mark Young

2021-03-26

Session 15 Article

(SI)

5.15 Session 16: Tissue Engineering for Organ Scaffolds

Daniel Bell

2021-03-29

Session 16 Article

(SI)

5.16 Session 17: Materials for Advanced Biological Imaging

Dr. Arda Gunay

2021-04-02

Session 17 Article

(SI)

5.17 Session 18: Microfabrication of Fate-Directing Scaffolds

Connor Miksch

2021-04-06

Session 18 Article

(SI)

5.18 Session 19: Medical Applications of Synthetic Networks

Bruce Kirkpatrick

2021-04-09

Session 19 Article

(SI)

5.19 Session 20: Synthetic Cancer Microenvironments

Della Shin

2021-04-16

Session 20 Article

(SI)

5.20 Session 21: Engineering Sex-Specific Cardiac Disease

Dr. Megan Schroeder

2021-04-20

Session 21 Article

(SI)

5.21 Session 22: Future Lab Directions

Dr. Kristi Anseth

2021-04-23

Topics covered

  • Future Lab directions