5 2021 Anseth Undergraduate Research Sessions
5.1 Session 2: Photoclick Hydrogels
Dr. Laura Macdougall
2021-01-26
(SI)
Topics covered
- Using absorbance and NMR to monitor reactant conversion and product formation
- In situ rheology, photocrosslinking, and photooxidation
- Irradiation requirements for transdermal vs. transparenchymal phototherapy
- Importance of validation on all Fitzpatrick skin types (avoiding racial bias in experimental design)
- Live/dead staining to verify cytocompatability (and limitations of this approach)
Key finding: Dihydrotetrazine can be photooxidized by methylene blue and red (625 nm) light. This oxidation converts dihydrotetrazine to tetrazine, which participates in an inverse electron-demand Diels-Alder addition to norbornene (one of many “click” reactions). Because this system is activated with red light (which can penetrate into tissues that aren’t accessible with transdermal UV light), the authors demonstrated both cytocompatbility and gelation through synthetic mimics of different skin types, indicating that this material has potential for use as an injectible and dynamically stiffening hydrogel.
Additional information
Oxidation-Induced “One-Pot” Click Chemistry. Albada et al. Chem Rev 2021.
5.2 Session 3: Matrix Cues for MSC Activation
Ali Borelli
2021-01-29
(SI)
Topics covered
- Using absorbance and NMR to monitor reactant conversion and product formation
- In situ rheology, photocrosslinking, and photooxidation
- Irradiation requirements for transdermal vs. transparenchymal phototherapy
- Importance of validation on all Fitzpatrick skin types (avoiding racial bias in experimental design)
- Live/dead staining to verify cytocompatability (and limitations of this approach)
Key finding: Dihydrotetrazine can be photooxidized by methylene blue and red (625 nm) light. This oxidation converts dihydrotetrazine to tetrazine, which participates in an inverse electron-demand Diels-Alder addition to norbornene (one of many “click” reactions). Because this system is activated with red light (which can penetrate into tissues that aren’t accessible with transdermal UV light), the authors demonstrated both cytocompatbility and gelation through synthetic mimics of different skin types, indicating that this material has potential for use as an injectible and dynamically stiffening hydrogel.
Additional information
Oxidation-Induced “One-Pot” Click Chemistry. Albada et al. Chem Rev 2021.
5.3 Session 4: Intestinal organoids in synthetic matrices
F. Max Yavitt
2021-02-01
(SI figures included in main text)
Topics covered
- Using absorbance and NMR to monitor reactant conversion and product formation
- In situ rheology, photocrosslinking, and photooxidation
- Irradiation requirements for transdermal vs. transparenchymal phototherapy
- Importance of validation on all Fitzpatrick skin types (avoiding racial bias in experimental design)
- Live/dead staining to verify cytocompatability (and limitations of this approach)
Key finding: Dihydrotetrazine can be photooxidized by methylene blue and red (625 nm) light. This oxidation converts dihydrotetrazine to tetrazine, which participates in an inverse electron-demand Diels-Alder addition to norbornene (one of many “click” reactions). Because this system is activated with red light (which can penetrate into tissues that aren’t accessible with transdermal UV light), the authors demonstrated both cytocompatbility and gelation through synthetic mimics of different skin types, indicating that this material has potential for use as an injectible and dynamically stiffening hydrogel.
Additional information
Oxidation-Induced “One-Pot” Click Chemistry. Albada et al. Chem Rev 2021.
5.4 Session 5: MSC secretome engineering
Varsha Rao
2021-02-01
(SI)
Topics covered
- Using absorbance and NMR to monitor reactant conversion and product formation
- In situ rheology, photocrosslinking, and photooxidation
- Irradiation requirements for transdermal vs. transparenchymal phototherapy
- Importance of validation on all Fitzpatrick skin types (avoiding racial bias in experimental design)
- Live/dead staining to verify cytocompatability (and limitations of this approach)
Key finding: Dihydrotetrazine can be photooxidized by methylene blue and red (625 nm) light. This oxidation converts dihydrotetrazine to tetrazine, which participates in an inverse electron-demand Diels-Alder addition to norbornene (one of many “click” reactions). Because this system is activated with red light (which can penetrate into tissues that aren’t accessible with transdermal UV light), the authors demonstrated both cytocompatbility and gelation through synthetic mimics of different skin types, indicating that this material has potential for use as an injectible and dynamically stiffening hydrogel.
Additional information
Oxidation-Induced “One-Pot” Click Chemistry. Albada et al. Chem Rev 2021.
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5.8 Session 9: Novel LCE Biomaterial Fabrication
Nat Skillin
2021-02-23
(SI)
Additional information
Voxelated liquid crystal elastomers. Ware et al. Science 2014
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